The acid process is the old and conventional method for changing the structure of carbohydrates and for using starch sources to produce alcohol. Due to the high temperature of this process, the use of acid and the failure to obtain glucose with high DE values, the acidic method has low efficiency and also damages the environment and pollutes it.
Use of enzymes is a modern and short-cut way of using the potential of cereals for alcohol production. This method employs alpha-amylase in the liquefaction stage and glucoamylase and pullulanase in the saccharification stage.
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